4) Add each 2 drops of extracted specimen from extraction tube to the rest three wells ( H2O2,SA,LE ,CV,TV well)
The kit is scientific to prepare on the basis of multi-test of, H2O2,sialidase (SA) and leuk-esterase (LE), candida vaginitis(CV), trichomonas vaginitis(TV) and pH value. These specific substrates will reacted with chromogenic reagent in the condition of enzyme.
(1).H2O2
H2O2reacts with peroxidase to produce nascent oxygen. With existence of 4-Aminoantipyrine, the nascent oxygen oxidizes 2,4,6-Trihydroxybenzoic acid to turn red. The intensity of red color is in proportion to H2O2concentration.
(2). Sialidase (SA)
The substrate BCIN (5-Bromo-4-chloro-3-indolyl -α-D-N-acetylneuraminic acid sodium), while reacting with neuramidinase (sialidase), will be decomposed into indoles, which will be oxidized to turn blue. The intensity of blue color is in proportion to sialidase activity level.
(3). Leukocyte esterase (LE)
The substrate BCIA (5-Bromo-4-chloro-3-indolyl -α-D-N-acetyl acetate), while reacting with leukocyte esterase; will be decomposed into indoles, which will be oxidized to show blue. The intensity of blue color is in proportion to leukocyte esterase activity level.
(4). Candida vaginitis(CV)
Candida has a strong NAG activity. The substrate reacts with it, will be decomposed into Nitrate phenylacetyl amino glucoside, release nitrophenol which will turn to yellow. The intensity of yellow color is in proportion to NAG activity level.
(5) Trichomonas vaginitis(TV)
The substrate will react with specific proteolytic enzyme in trichomonas. Which will hydrolysis in a short time and appear light blue or blue is positive result, if no color change is negative result. The intensity of blue color is in proportion to quantity of trichomonas.
6).PH value
pH value test is based on the principle of acid - base indicators. Yellow, greenish yellow or green color indicate pH value ≤4.5, whereas greenish blue or blue indicate pH value >4.5
