The Neisseria Gonorrhoeae antigen test kit is used to detect N. Gonorrhoeae through visual interpretation of the color development in the internal strip. The membrane was immobilized with gonococcal Antigen-specific polychonal antibody on the test position (T) and related antibodies on the control position(C).

During testing, the specimen is added to the sample well (S) and reacts with anti-gonococcus monoclonal antibody conjugated to colored polystyrene latex microsphere and precoated onto the sample pad of the test. Then, the mixture migrates through the membrane by capillary effect and interacts with immobilized monoclonal antibody on the membrane. If there is sufficient gonococcus antigens in the specimen, a colored band will form at the test well (T) of the membrane. This colored band indicates a positive result, while its absence indicates a negative result. A colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.